prehybridized

动词 v.

英文释义

动词 v.
  1. simple past and past participle of prehybridize form-of,participle,past
    — The membranes were prehybridized with Hyb TM Plus buffer (Sigma) for 4 h followed by hybridization with the DNA probes overnight at 50°C. After washing the membranes twice with 5X SSC and once with 1X SSC and 0.1% SDS at 50°C, they were exposed for 2 days to radioactive sensitive screens, and scanned using a Thyphoon Trio scanner (AmershamBiosciences, New Jersey, USA).
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