multichromatogram

— Initially, we set the following 2 criteria to determine the concentration of a protein: 1) the retention times of peaks in multichromatograms of transitions should be the same between endogenous peptide, thereby confirming that the peptide originated from the target protein; and 2) the transition should have an average peak height for endogenous peptide above 1000 cps in the massive lymph node metastasis group.