irresponsiveness

— As per the guidelines, all animals with severe CS were treated with clinically appropriate anticonvulsants, and mandatory euthanization (with pentobarbital anesthesia) was performed if animals exhibited severe, refractory CS and/or presented in poor physical condition, defined as persistent immobility, lack of eating or drinking, irresponsiveness to touch, loss of the righting reflex, and/or a substantial reduction in body weight (≥20% of baseline level).